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Tuesday, August 11, 2020 | History

2 edition of Microencapsulation of viable human fibroblasts in a polyacrylate hydrogel. found in the catalog.

Microencapsulation of viable human fibroblasts in a polyacrylate hydrogel.

Alexander Harvey Boag

Microencapsulation of viable human fibroblasts in a polyacrylate hydrogel.

by Alexander Harvey Boag

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  • 25 Currently reading

Published .
Written in English


The Physical Object
Pagination89 leaves
Number of Pages89
ID Numbers
Open LibraryOL18822955M

  Therefore, hydrogel properties must be matched to specific cell candidates in order to establish viable cytocompatible cell microencapsulation platforms. The high long-term survival rate of these cells, selected for their utility across a range of tissue engineering and regenerative medicine applications, would facilitate downstream. Sommar P, Pettersson S, Ness C, Johnson H, Kratz G, Junker JPE. Engineering three-dimensional cartilage- and bonelike tissues using human dermal fibroblasts and macroporous gelatine microcarriers. J Plast Reconstr Aesthet Surg. ;– CrossRef Google Scholar.

  Natural polymers for the microencapsulation of cells. Journal of The Royal Society Interface , 11, DOI: /rsif Susana G. Guerreiro, Maria J. Oliveira, Mário A. Barbosa, Raquel Soares, Pedro L. Granja. Neonatal Human Dermal Fibroblasts Immobilized in RGD–Alginate Induce Angiogenesis.   With human fibroblasts, the encapsulation process resulted in % dead cells (at Day 0). With HUVECs, which are more sensitive cells, the encapsulation resulted in % dead cells. Between Days 0 and 1, the ratio of dead fibroblasts had increased by % and from Day 1 to Day 2, the ratio of dead cells increased a further %.

  ATCC® Normal Adult Human Primary Dermal Fibroblasts, when grown in Fibroblast Basal Media supplemented with Fibroblast Growth Kit components, provide an ideal cell system to propagate dermal fibroblasts in either serum-free or low serum conditions. The cells are cryopreserved in their first passage to ensure the highest viability and plating efficiency.   They were able to culture Lifeline® primary neonatal human fibroblasts in both 2D and 3D hydrogel culture systems using our FibroLife® S2 medium and reported over 95% cell viability. Furthermore, the authors were able to modify the properties of their hydrogels by altering the protein polymer compositions to include bioactive components that.


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Microencapsulation of viable human fibroblasts in a polyacrylate hydrogel by Alexander Harvey Boag Download PDF EPUB FB2

Viable human diploid fibroblasts have been micro‐encapsulated in EUDRAGIT RL, a commercially available water‐insoluble polyacrylate, by an interfacial precipitation technique.

Cells in medium and polymer solution (in diethyl phthalate) were coextruded and formed into droplets by a coaxial air by:   It may also ' Butterworth & Co (Publishers) Ltd. 2/89/$ BiomaterialsVol 10 August Microencapsulation of human diploid fibroblasts: C.L.

Mallabone et al. need to be permeable to growth factors or components in serum, if these are consumed during cell by:   The islet suspension was drawn up into ~ l m of polyethylene tubing (Intramedic PE) using a syringe which was then used directly in the microencapsulation apparatus.

Human diploid fibroblasts (HDF) were derived from a skin biopsy at the Hospital for Sick Children, Toronto, and used between the 10th and 30th by:   Successful results using AN69 were also achieved in a degenerative photoreceptor model. Uteza et al. 38 genetically engineered mouse fibroblasts (NIH 3T3) to express human fibroblast growth factor (hFGF‐2) and then encapsulated the cells within AN69 hollow microfibers.

The loaded microfibers were implanted into the vitreous cavity of the eyes Cited by:   Although we briefly highlight some polymers and their uses for cell microencapsulation in this review, it should be noted that some polymers have been used for the encapsulation of non-stem cells (e.g., fibroblasts, myoblasts, and hepatocytes) for the purpose of studying fundamental cell-material interactions, drug screening, or tissue Cited by:   J.

and Sugamori, M.E., Microencapsulation of mammalian cells in a water insoluble polyacrylate by coextrusion and interfacial precipitation. Biotech. Bioeng. 11 1 BiomaterialsVol 8 September CHO cells in HEMA-MMA- R.M. Dawson et al 10 Boag, A.H. and Sefton, M.V., Microencapsulation of human fibroblasts in a water.

Uteza Y, Rouillot J-S, Kobetz A, Marchant D, Pecqueur S, Arnaud E, Prats H, Honiger J, Dufier J-L, Abitbol M. Intravitreous transplantation of encapsulated fibroblasts secreting the human fibroblast growth factor 2 delays photoreceptor cell degeneration in Royal College of Surgeons rats.

Proc Natl Acad Sci USA. ; –   Boag A H and Sefton M V Microencapsulation of human-fibroblasts in a water Dawson R M, Broughton R L, Blysniuk J and Sugamori M E Microencapsulation of mammaliancells in a water-insoluble polyacrylate by coextrusion Ranjit S, Mondol R, Ray S and Sa B Al(+3) ion cross-linked and acetalated gellan hydrogel network beads.

Aikawa, in Comprehensive Biomaterials, Fibroblasts. Fibroblasts are critical components of granulation tissue. Fibroblast accumulation begins 3–5 days after injury and may last up to 14 days.

Following biomaterial implantation, fibroblasts undergo a response known as ‘activation,’ characterized by a transition of quiescent cells into myofibroblast-like phenotype.

Microbead generation with encapsulated NIH-3T3 cells. NIH-3T3 fibroblast cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Sigma-Aldrich) supplemented with heat-inactivated fetal bovine serum (10%, FBS, Gibco) and penicillin-streptomycin (1%, Gibco) at 37 °C, in a humidified atmosphere with 5% of CO 2.A cell suspension (3×10 6 cells/mL) was prepared by trypsinizing NIH.

Acta () 7 A.H. Boag and M. Sefton, Microencapsulation of hu- man fibroblasts in a water-insoluble polyacrylate, Bio- technol.

Bioeng. 30 () 8 M.E. Sugamori and M. Sefton, Microencapsulation of pancreatic islets in a water insoluble polyacrylate, Transactions ASAIO 35 () 9 J.A. Douglas and M.V. Microencapsulation of human fibroblasts in a water insoluble polyacrylate.

Black JP, Sefton MV. Viability and protein secretion from human hepatoma (HepG2) cells encapsulated in fini polyacrylate microcapsules by submerged nozzle-liquid jet extrusion. Wells GDM, Fisher MM, Sefton MV.

Microencapsulation of viable. Microencapsulation also improved the culture of hESC aggregates by protecting cells from hydrodynamic shear stress, controlling aggregate size and maintaining cell pluripotency for two weeks. This work establishes that microencapsulation technology may prove a powerful tool for integrating the expansion and cryopreservation of pluripotent hESCs.

Microencapsulation of bioactive agents, mixed with the GG hydrogel solution and used to produce encapsulated pseudo-islets using the proposed system.

Boag A H and Sefton M V Microencapsulation of human-fibroblasts in a water-insoluble polyacrylate Biotechnol. Bioeng. 30 – Crossref Google Scholar. Microencapsulation is widely use in industry but remains relatively unknown from the public.

The reason is that microcapsules are not an end- product, but generally a technique to overcome process. Cell Encapsulation in Alginate Microcapsule by Emulsion Cross-Linking Technique.

Human fibroblast cells at a concentration of 5x[sup.5] cells/ml were placed in 1% (w/v) sodium alginate solution and transferred to the oil solution and mixed for 10 minutes. Microfluidic-Based Synthesis of Hydrogel Particles for Cell Microencapsulation and Cell-Based Drug Delivery Article (PDF Available) in Polymers 4(4) December with Reads.

hydrogel that has been used in soft contact lens es, human fibroblasts, hum an erythrocytes, rat islet cells, for the microencapsulation of fibroblasts or pancreatic islets.

J Control Release. At present, living cells are widely used in cell transplantation and tissue engineering. Many efforts have been made aiming towards the use of a large number of living cells with high activity and integrated functionality.

Currently, cryopreservation has become well-established and is effective for the long-term storage of cells. However, it is still a major challenge to inhibit cell damage. Lee W 1, Debasitis J C, Lee V K, Lee J H, Fischer K, Edminster K, Park J K and Yoo S S Multi-layered culture of human skin fibroblasts and keratinocytes through three-dimensional freeform fabrication Biomaterials 30 – Crossref.

Encapsulation of fibroblasts in 2 and 5% w/v alginate hydrogel effectively inhibited their proliferation, whilst maintaining cell viability allowing keratinocytes to grow uninhibited by fibroblast.With human fibroblasts, the encapsulation process resulted in % dead cells (at Day 0).

With HUVECs, which are more sensitive cells, the encapsulation resulted in % dead cells. Between Days 0 and 1, the ratio of dead fibroblasts had increased by % and from Day 1 to Day 2, the ratio of dead cells increased a further %.

Fibroblasts can express vascular endothelial growth factor (VEGF), which plays a major role in angiogenesis and also acts as a chemoattractant and survival factor for CMs and cardiac progenitors.

In this in vitro model study, mouse NIH 3T3 fibroblasts encapsulated in 2% w/v Ca-alginate were shown to remain viable for days.